Jain, Aviral and Chasoo, Gousia and Singh, Shashank K. and Saxena, Ajit K. and Jain, Sanjay K. (2011) Transferrin-appended PEGylated nanoparticles for temozolomide delivery to brain:in vitrocharacterisation. Journal of Microencapsulation, 28 (1). pp. 21-28. ISSN 0265-2048

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Polymer-based nanotechnologies are proposed to be an alternative for drug administration, delivery and targeting to those of conventional formulations. The blood brain barrier is frequently a rate-limiting factor in determining permeation of a drug into brain. In this study, the surface-engineered long-circulating PLGA nanoparticles (NPs) were assessed for brain-specific delivery. Long circulating NPs of PLGA- and PEG-synthesised copolymer were prepared by emulsification solvent evaporation method. Further, the surface of PEGylated NPs was modified by anchoring transferrin (Tf) ligand for receptor-mediated targeting to brain. NPs were characterised for shape and size, zeta potential, entrapment efficiency and in vitro drug release. In vitro cytotoxicity studies were performed on human cancer cell lines. Confocal Laser Scanning Microscopy studies show the enhanced uptake of Tf-appended PEGylated NPs and their localisation in the brain tissues. Hence, the specific role of Tf ligand on PEGylated NPs for brain delivery was confirmed.

Item Type: Article
Subjects: Pharmacological Sciences
Depositing User: Mr. Amit Nargotra
Date Deposited: 03 Jan 2012 05:28
Last Modified: 03 Jan 2012 05:28
URI: http://iiim.csircentral.net/id/eprint/364

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