Khan, Imran and Sangwan, Payare L. and Abdullah, Sheikh Tasduq and Gupta, Bhisan D. and Dhar, Jagdish K. and Manickavasagar, Rajendran and Koul, Surrinder (2011) Ten marker compounds-based comparative study of green tea and guava leaf by HPTLC densitometry methods: Antioxidant activity profiling. Journal of Separation Science, 34 (7). pp. 749-760. ISSN 16159306

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Abstract

High-performance thin layer chromatography (HPTLC) method for the separation and quantitative determination of ten markers (catechins, flavonoids, and phenolics) in different extracts of green tea and guava leaf has been developed and the antioxidant activity profiles of the two plant extracts have been determined. Ten marker compounds have been resolved using silica gel 60 F254 plates, toluene/acetone/formic acid (5:4:1 v/v/v)for markers 1–6, and toluene/ethyl acetate/formic acid/methanol (3:3:0.8:0.2 v/v/v/v) for markers 7–10 as the mobile phases. The high-performance thin layer chromatography densitometry was performed at wavelengths of 282 and 285 nm for the markers 1–6 and 7–10, respectively. Potent antioxidant activity and the presence of phenolics and flavan-3-ols has been observed for the guava leaf extracts suggestive of its use as an alternate economical source of antioxidants over green tea – the well-established food additive/ nutraceutical agent.

Item Type:	Article
Subjects:	Chemical Sciences
Divisions:	UNSPECIFIED
Depositing User:	Mr. Amit Nargotra
Date Deposited:	03 Jan 2012 05:23
Last Modified:	03 Jan 2012 05:23
URI:	http://iiim.csircentral.net/id/eprint/360

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