Thind, Tarunpreet Singh and Agrawal, Satyam Kumar and Saxena, A.K. and Arora, Saroj (2008) Studies on cytotoxic, hydroxyl radical scavenging and topoisomerase inhibitory activities of extracts of Tabernaemontana divaricata (L.) R.Br. ex Roem. and Schult. Food and Chemical Toxicology, 46 (8). pp. 2922-2927. ISSN 02786915

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In the present investigation, the cytotoxic, hydroxyl radical scavenging and topoisomerase inhibition activities of Tabernaemontana divaricata (Apocynaceae) were evaluated. The extracts from leaves of the plant were prepared with different solvents viz. chloroform, methanol, ethyl acetate and hexane. In, invitro cytotoxicity assay, with cell lines viz HCT-15 (Colon), HT-29 (Colon), 502713 (Colon), MCF-7 (Breast), PC- 3 (Prostrate), it was observed that the ethyl acetate extract was effective against only onecolon cell line (502713) at the lowest dose i.e. 10 lg/ml, whereas the chloroform extract was effective against all the three colon cancer cell lines, at 30 lg/ ml. In order to evaluate the mechanism of cytotoxicity of these extracts, they were assessed for their ability to scavenge hydroxyl radicals in plasmid nicking assay with pBR322. It was observed that all the extracts effectively inhibited the unwinding of supercoiled DNA except hexane extract, which showed the least effect. Since the expression of topo enzymes is linked with cell proliferation so the extracts were also checked for topo I and topo II inhibitory activities. It was noticed that ethyl acetate extract selectively showed inhibition of topo II in topoisomerase II relaxation assay.

Item Type: Article
Subjects: Pharmacological Sciences
Depositing User: Mr. Amit Nargotra
Date Deposited: 03 Jan 2012 04:56
Last Modified: 03 Jan 2012 04:56

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