Simultaneous high-performance liquid chromatographic determination of Cedrus deodara active constituents and their pharmacokinetic profile in mice

Sachin, B.S. and Koul, M. and Zutshi, A. and Singh, S.K. and Tikoo, A.K. and Tikoo, M.K. and Saxena, A.K. and Sharma, S.C. and Johri, R.K. (2008) Simultaneous high-performance liquid chromatographic determination of Cedrus deodara active constituents and their pharmacokinetic profile in mice. Journal of Chromatography B, 862 (1-2). pp. 237-241. ISSN 15700232

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Official URL: http://dx.doi.org/10.1016/j.jchromb.2007.12.015

Abstract

A specific and sensitive high-performance liquid chromatographic (HPLC) method with photodiode-array (PDA) ultraviolet detection was developed for the simultaneous determination of three bioactive constituents of Cedrus deodara namely wikstromol, matairesinol and dibenzylbutyrolactol in mouse plasma. In solid-phase extraction (SPE) these constituents were successfully separated using a C18 column by isocratic elution using acetonitrile:water containing hexanesulphonic acid, 32:68 (v/v). The flow rate was set at 1 ml/min and detector wavelength at 225 nm. Good linearity (r2 > 0.999) was observed over the studied range of 0.015–5.0 �g/ml for wikstromol and 0.030–5.0 �g/ml for matairesinol and dibenzylbutyrolactol.The CV values of intra-day precision for wikstromol, matairesinol and dibenzylbutyrolactol were in between 1.8–6.9, 1.7–4.9 and 1.6–4.2% and values of inter-day precision were in between 10.4–12.2, 9.7–11 and 10–11.2%, respectively. The extraction recoveries at low to high concentration were greater than 98, 83 and 87% for each analyte, respectively. The LOQ for wikstromol was 0.015 �g/ml and for both matairesinol and dibenzylbutyrolactol it was 0.030 �g/ml. The developed method was used to determine the pharmacokinetics of the three analytes in mice after intraperitoneal administration of CD-3.

Item Type:	Article
Subjects:	Chemical Sciences Pharmacological Sciences
Divisions:	UNSPECIFIED
Depositing User:	Mr. Amit Nargotra
Date Deposited:	02 Jan 2012 12:15
Last Modified:	02 Jan 2012 12:15
URI:	http://iiim.csircentral.net/id/eprint/342

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