Khajuria, Ravi K. and Suri, Krishan A. and Gupta, Rajinder K. and Satti, Naresh K. and Amina, Musarat and Suri, Om P. and Qazi, Ghulam N. (2004) Separation, identification, and quantification of selected withanolides in plant extracts ofWithania somnifera by HPLC-UV(DAD) - Positive ion electrospray ionisation-mass spectrometry. Journal of Separation Science, 27 (7-8). pp. 541-546. ISSN 1615-9306

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Abstract

This paper describes a method for separation, identification, and quantification of selected withanolides in Withania somnifera plant extracts by HPLC-UV(DAD)–Mass Spectrometry (HPLC-MS). Withaferin-A (WS-3), 12-deoxywithastramonolide (WS-12DS), Withanolide A (WS-1), and Withanone (WS-2) were used as external standards. The compounds were isolated from Withania somnifera by repeated column chromatography of the root extract and their identity was established by 1H- and 13CNMR and mass spectral data. The compounds were chromatographed on a Merck(25064.6 mm ID, 5 lm) column and analyzed by Electrospray Ionization on a mass spectrometer in Selected Ion Mode (SIM). For quantification, [M + Na]+ ions were monitored. Linear calibration curves were obtained in the concentration range of 1.50 lg/mL to 6.5 lg/mL. The method was applied successfully to the detection and quantification of the said withanolides in a number of samples.

Item Type:	Article
Subjects:	Chemical Sciences
Divisions:	UNSPECIFIED
Depositing User:	Mr. Amit Nargotra
Date Deposited:	02 Jan 2012 12:14
Last Modified:	02 Jan 2012 12:14
URI:	http://iiim.csircentral.net/id/eprint/340

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