Bani, Sarang and Gautam, Manish and Sheikh, Fayaz Ahmad and Khan, Beenish and Satti, Narender Kumar and Suri, Krishan Avatar and Qazi, Ghulam Nabi and Patwardhan, Bhushan (2006) Selective Th1 up-regulating activity of Withania somnifera aqueous extract in an experimental system using flow cytometry. Journal of Ethnopharmacology, 107 (1). pp. 107-115. ISSN 03788741

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Withania somnifera (Ashwagandha) is reported to be immunoprotective and immunoadjuvant. We studied its roots aqueous extract on T helper(Th) immunity using flowcytometry. This extractwas standardized with six withanolides as marker compounds using HPLC. Once daily dose ranging from 25 to 400 mg/kg/p.o. was used to study effect on Th1: IFN-gamma, IL-2 and Th2: IL-4 cytokine modulation. We also studied effect on CD4 and CD8 in normal and immunesuppressed mice. The results indicate that extract at 100 mg/kg resulted significant selective up-regulation of Th1 response.Treatment with extract showed significant increase inCD4andCD8counts as compared to control and cyclopsorin A, with a faster recovery of CD4+ T cells in immunesuppressed animals. Under immunesuppressed conditions, potentiation of cellular and humoral immune responses of extract was comparable to levamisole. This study indicates the selective Th1 up-regulating effect of extract and suggests its use for selective Th1/Th2 modulation.

Item Type: Article
Subjects: Chemical Sciences
Pharmacological Sciences
Depositing User: Mr. Amit Nargotra
Date Deposited: 02 Jan 2012 12:12
Last Modified: 02 Jan 2012 12:12

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