Vakhlu, J. and Johri, S. and Verma, V. and Koul, S. and Parshad, R. and Taneja, S.C. and Qazi, G.N. (2005) Purification and properties of enantioselective ester hydrolase from a strain of Trichosporon species (DSMZ 11829). Enzyme and Microbial Technology, 37 (3). pp. 330-339. ISSN 01410229

PDF - Published Version Restricted to Registered users only Download (297Kb) | Request a copy

Abstract

An isolated strain of Trichosporon species (DSMZ 11829) was capable of producing a novel membrane-bound, enantioselective ester hydrolase. Its application in the kinetic resolution of racemic methyl ester of 6-methoxy-�-methyl-2-naphthaleneacetic acid (naproxen) into (S)-(+)-naproxen (>99% ee, E∼500) and acyloxy derivatives of 1-chloro-3-(1-naphthyloxy)-2-propanol into (R)-alcohol (>99% ee, E∼550) have already been reported by us. The enzyme designated as TSL was extracted from the cells by toluenization and purified to homogeneity from cell free extract with 8.5% overall yield. The purified enzyme with a specific activity of 831 U/mg protein was achieved by column chromatography using DEAE-Sepharose, phenyl-Sepharose and Sephadex G-100 resins. The purified enzyme exhibited hydrolytic activity without any noticeable decrease in the rate of hydrolysis or its enantioselectivty in the resolution of racemic naproxen into (S)-(+)-naproxen or in the resolution of 1-chloro-3-(1-naphthyloxy)-2-propanol and 1-(6-methoxy-2-naphthyl)ethanol. Purified enzyme is a glycosylated monomer with a molecular weight of∼40 kD and isoelectric point (pI)∼4.1. TSLwas maximally active at 30 ◦C and pH 8.0. The enzymewas insensitive to serine reactive agent PMSF, metal chealator EDTA, non-ionic detergent Triton X-100 and reducing agent mercaptoethanol. The N-terminal amino acid sequence of TSL determined as Thr-Val-Thr-Thr-Pro-Thr-Leu-Ser-Ala-Asp-Ala-Lys-Lys-Gly- did not reveal any homology with the N-terminal amino acid sequence of any other known microbial ester hydrolases.

Item Type:	Article
Subjects:	Biological Sciences
Divisions:	UNSPECIFIED
Depositing User:	Mr. Amit Nargotra
Date Deposited:	02 Jan 2012 12:01
Last Modified:	02 Jan 2012 12:01
URI:	http://iiim.csircentral.net/id/eprint/322

Actions (login required)

View Item