Ahmad, Nasier and Johri, Sarojini and Abdin, Malik Z. and Qazi, Ghulam N. (2009) Molecular characterization of bacterial population in the forest soil of Kashmir, India. World Journal of Microbiology and Biotechnology, 25 (1). pp. 107-113. ISSN 0959-3993

PDF - Published Version Restricted to Registered users only Download (226Kb) | Request a copy

Abstract

The bacterial diversity in the forest soil of Kashmir, India was investigated by 16S rDNA-dependent molecular phylogeny. Small subunit rRNA (16S rDNA)from forest soil metagenome were amplified by polymerase chain reaction (PCR) using primers specific to the domain bacteria. 30 unique phylotypes were obtained by PCR based RFLP of 16S rRNA genes using endonucleases Hae 111 and Msp 1, which were most suitable to score the genetic diversity. The use of 16S rRNA analysis allowed identification of several bacterial populations in the soil belonging to the following phyla: Firmicutes (33.3%),Bacteroidetes (13.3%), Proteobacterium (6.6%), Planctomycete(3.3%), and Deferribacteraceae (3.3%) in addition to the others that were not classified, beyond Archaea domain, However, 36.6% of the retrieved bacterial sequences could not be grouped with any phylum/lineage.The large amount of unclassified clone sequence could imply that novel groups of bacteria were present in the forest soil.

Item Type:	Article
Subjects:	Biological Sciences
Divisions:	UNSPECIFIED
Depositing User:	Mr. Amit Nargotra
Date Deposited:	02 Jan 2012 10:50
Last Modified:	02 Jan 2012 10:50
URI:	http://iiim.csircentral.net/id/eprint/294

Actions (login required)

View Item