Singh, B. and Chandan, B. K. and Sharma, N. and Bhardwaj, V. and Satti, N. K. and Gupta, V. N. and Gupta, B. D. and Suri, K. A. and Suri, O. P. (2006) Isolation, structure elucidation andIn Vivo hepatoprotective potential oftrans-tetracos-15-enoic acid from Indigofera tinctoria Linn. Phytotherapy Research, 20 (10). pp. 831-839. ISSN 0951-418X

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Abstract

The bioassay guided fractionation of the dried aerial part of Indigofera tinctoria Linn. led to the identification of an active fraction labelled as indigotin. On further chemical analysis, a compound isolated from indigotin was identified and characterized as trans-tetracos-15-enoic acid (TCA). The chemical structure of this compound was established on the basis of physical properties and spectral data, including NMR. It afforded significant hepatoprotection against carbon tetrachloride and paracetamol induced hepatotoxicity in experimental models. Silymarin, a well known plant based hepatoprotective agent, and N-acetylcysteine, which has proven efficacy as a replenisher of sulfhydryls, were used for relative efficacy. TCA was found to reverse the altered hepatic parameters in experimental liver damage. In the safety evaluation study the oral LD50 was found to be more than 2000 mg/ kg, with no signs of abnormalities or any mortality for the 15 day period of observation after administration of a single dose of drug in mice. The studies revealed significant and concentration dependent hepatoprotective potential of TCA as it reversed the majority of the altered hepatic parameters in experimental liver damage in rats and mice and may be useful in the management of liver disorders.

Item Type:	Article
Subjects:	Chemical Sciences Pharmacological Sciences
Divisions:	UNSPECIFIED
Depositing User:	Mr. Amit Nargotra
Date Deposited:	28 Dec 2011 11:45
Last Modified:	28 Dec 2011 11:45
URI:	http://iiim.csircentral.net/id/eprint/285

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