Kaur, Amandeep and Singh, Jatinder and Kamboj, Sukhdev Singh and Sexana, A.K. and Pandita, Renu Moti and Shamnugavel, M. (2005) Isolation of an N-acetyl-d-glucosamine specific lectin from the rhizomes of Arundo donax with antiproliferative activity. Phytochemistry, 66 (16). pp. 1933-1940. ISSN 00319422

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Abstract

A lectin with antiproliferative activity towards human cancer cell lines and mitogenic towards human peripheral blood mononuclear cells was purified from the rhizomes of Arundo donax (Linn.) by affinity chromatography on N-acetyl-D-glucosamine linked to epoxy-activated sepharose-6B. The pure preparation apparently yielded a single band of approximately 15 kDa on SDS–PAGE,pH 8.3, under both reducing and non-reducing conditions. The molecular mass of native lectin was 32 kDa as determined by gel filtration chromatography. This showed the lectin to be a dimer, with subunits not held together by disulphide linkages. The A. donax lectin (ADL) agglutinated rabbit erythrocytes and the agglutination was inhibited by N-acetyl-D-glucosamine and its diand trimer. The lectin was thermostable upto 55 �C and showed optimum activity in the range of pH 7.0–9.0 and comprised of 2.1% carbohydrate content.

Item Type:	Article
Subjects:	Chemical Sciences
Divisions:	UNSPECIFIED
Depositing User:	Mr. Amit Nargotra
Date Deposited:	28 Dec 2011 11:34
Last Modified:	28 Dec 2011 11:34
URI:	http://iiim.csircentral.net/id/eprint/282

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