Kaur, Amandeep and Kamboj, Sukhdev Singh and Singh, Jatinder and Saxena, A. K. and Dhuna, Vikram (2005) Isolation of a Novel N-acetyl-d-lactosamine Specific Lectin from Alocasia cucullata (Schott.). Biotechnology Letters, 27 (22). pp. 1815-1820. ISSN 0141-5492

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An N-acetyl-D-lactosamine (LacNAc) specific lectin from tubers of Alocasia cucullata was purified by affinity chromatography on asialofetuin-linked amino activated silica. The pure lectin showed a single band in SDS-PAGE at pH 8.8 and was a homotetramer with a subunit molecular mass of 13.5 kDa and native molecular mass of 53 kDa. It was heat stable up to 55 �C for 15 min and showed optimum hemagglutination activity from pH 2 to 11. The lectin was affected by denaturing agents such as urea (2 M), thiourea (2 M) and guanidine�HCl (0.5 M) and did not require Ca2+ and Mn2+ for its activity. It was a potent mitogen at 10 lg/ml towards human peripheral blood mononuclear cells with 50% growth inhibitory potential towards SiHa (human cervix ) cancer cell line at 100 lg/ml.

Item Type: Article
Subjects: Biological Sciences
Depositing User: Mr. Amit Nargotra
Date Deposited: 28 Dec 2011 11:33
Last Modified: 28 Dec 2011 11:33
URI: http://iiim.csircentral.net/id/eprint/281

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