Verma, Monika and Singh, Shashank and Bhushan, Shashi and Pal, Harish and Kitchlu, Surinder and Koul, Maharaj and Thappa, Rajender and Saxena, Ajit (2008) Induction of Mitochondrial-Dependent Apoptosis by an Essential Oil from Tanacetum gracile. Planta Medica, 74 (5). pp. 515-520. ISSN 0032-0943

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Abstract

The essential oil of Tanacetum gracile (Accession no. AT-01 termed AT-01 in the manuscript), a cold desert alpine highly aromatic herb, has 40 constituents including lavendulol (21.5%), lavendulol acetate (1.7 %), α-pinene (11.2%),1,8-cineole (15.2 %), cis-β-ocimene (6.9 %), borneol (6.1%), limonene (5.1%) and chamazulene (3.7 %). AT-01 was evaluated for its anticancer activity. It inhibited HL-60 cell proliferation with an IC50 of 27 μg/ mL. Furthermore, AT-01 induced apoptosis in human leukemia HL-60 cells as measured by several biological end points. AT-01 induced apoptotic body formation, enhanced annexinV-FITC binding of the cells, increased sub-G0 DNA fraction, loss of mitochondrial membrane potential (Δψmt) and release of cytochrome c from mitochondria, activated caspase-9 as well as caspase-3, and increased cleavage of PARP in HL-60 cells. Thus, AT-01 induced apoptosis through the mitochondrial dependant pathway in HL-60 cells.

Item Type:	Article
Subjects:	Biological Sciences Pharmacological Sciences
Divisions:	UNSPECIFIED
Depositing User:	Mr. Amit Nargotra
Date Deposited:	28 Dec 2011 09:48
Last Modified:	28 Dec 2011 09:48
URI:	http://iiim.csircentral.net/id/eprint/278

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