Koul, Surrinder and Parshad, Rajinder and Taneja, Subhash C. and Qazi, Ghulam N. (2003) Enzymatic resolution of naproxen. Tetrahedron: Asymmetry, 14 (16). pp. 2459-2465. ISSN 09574166

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Trichosporon sp. (TSL), a newly found strain isolated from a locally fermented cottage cheese has been found to be highly stereoselective in the resolution of(S)-(+)-naproxen (ee >99%, E�500) from the corresponding racemic methyl ester. The process of resolution using whole cells has been scaled up to multi-kg level. Optimization of experimental conditions including downstream processing at 80–100 g/L substrate concentration with >90% recovery has been achieved. Changes in the physical parameters such as the particle size of the substrate play an important role in the resolution kinetics. A new strain of Trichosporon sp. having high cell density in cultivation (>60 g dry cell mass L−1 in 14–16 h) is found to be sufficiently stable for two years in dry powder form at 5–8°C. The viability of the resolution process has been further improved by the development of a simple racemization process for the enriched (R)-(−)-ester.

Item Type: Article
Subjects: Chemical Sciences
Depositing User: Mr. Amit Nargotra
Date Deposited: 28 Dec 2011 08:58
Last Modified: 28 Dec 2011 08:58
URI: http://iiim.csircentral.net/id/eprint/251

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