Chaubey, Asha and Parshad, Rajinder and Taneja, Subhash C. and Qazi, Ghulam N. (2009) Arthrobacter sp. lipase immobilization on magnetic sol 13gel composite supports for enantioselectivity improvement. Process Biochemistry, 44 (2). pp. 154-160. ISSN 13595113

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A bacterial lipase from Arthrobacter sp. (ABL: IIIM Jammu, India strain, MTCC No. 5125) has been immobilized on non-magnetic (Type A) and magnetic (Type B) supports derived from copolymerization of 3-aminopropyltriethoxysilane and tetraethylorthosilicate.Immobilized ABLpresented 21 1334 mg/g protein binding providing 30 1375 units/g activity in Type A non-magnetic composites and 24 1345 mg/g protein binding providing 35 1390 units/g activity with Type B supports containing magnetic particles. Immobilized ABL preparations have shownenhanced stability at pH5 139 and temperature up to 70 8C whereas free ABL is unstable under these conditions. Improved hydrolytic conversion as well as enantioselectivity were observedwith acylfluoxetine intermediate (ethyl3-hydroxy-3-phenylpropanoate alkyl acylates) andchiral auxiliaryacyl 1-phenyl ethanol usingimmobilized ABL derivatives (ee�99%; 3 134-fold increase in E-values) as compared to ABL enzyme/cells (ee 93 1398%). Introduction of magnetic particles in these supports has led to easier separation process with high product recovery yields.

Item Type: Article
Subjects: Biological Sciences
Depositing User: Mr. Amit Nargotra
Date Deposited: 28 Dec 2011 08:09
Last Modified: 28 Dec 2011 08:09

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