Chaubey, Asha and Parshad, Rajinder and Koul, Surrinder and Taneja, Subhash C. and Qazi, Ghulam N. (2006) Arthrobacter sp. lipase immobilization for improvement in stability and enantioselectivity. Applied Microbiology and Biotechnology, 73 (3). pp. 598-606. ISSN 0175-7598

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Abstract

Arthrobacter sp. lipase (ABL, MTCC no. 5125)is being recognized as an efficient enzyme for the resolution of drugs and their intermediates. The immobilization of ABL on various matrices for its enantioselectivity,stability, and reusability has been studied. Immobilization by covalent bonding on sepharose and silica afforded a maximum of 380 and 40 IU/g activity, respectively,whereas sol 13gel entrapment provided a maximum of 150 IU/g activity in dry powder. The immobilized enzyme displayed excellent stability in the pH range of 4 1310 and even at higher temperature, i.e., 50 1360

Item Type:	Article
Subjects:	Biological Sciences
Divisions:	UNSPECIFIED
Depositing User:	Mr. Amit Nargotra
Date Deposited:	28 Dec 2011 08:07
Last Modified:	28 Dec 2011 08:07
URI:	http://iiim.csircentral.net/id/eprint/218

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