Sharma, S. N. and Johri, R. K. and Sharma, S. C. and Suri, K. A. and Gupta, B. D. and Khan, I. and Sharma, P. and Sharma, V. K. and Dutt, P. and Amina, M. and Satti, N. K. (2009) A simple and reliable preparative high-performance liquid chromatographic technique for isolation of a bioactive flavone diglycoside from and extract of <i>Cuminum cyminum</i> seeds. Acta Chromatographica, 21 (3). pp. 499-512. ISSN 1233-2356

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Abstract

In this paper we describe a sensitive and reproducible reversed-phase highperformance liquid chromatography (HPLC) method with photodiode-array detection for isolation and quantification of the bioactive hydrophilic constituent 7-(1-O-β-D-galacturonide-4′-(1-O-β-D-glucopyranosyl)-3′,4′,5,7-tetrahydroxyflavone, 1, from the seeds of Cuminum cyminum. Compound 1 was separated isocratically on a C18 preparative column, in high purity, after removal of solvents. The purity and identity of the compound were established by use of LC–mass spectrometry and by spectroscopic techniques(1H and 13C NMR). The purity of 1 was also confirmed by HPTLC.

Item Type:	Article
Subjects:	Chemical Sciences
Divisions:	UNSPECIFIED
Depositing User:	Mr. Amit Nargotra
Date Deposited:	19 Dec 2011 08:12
Last Modified:	19 Dec 2011 08:12
URI:	http://iiim.csircentral.net/id/eprint/195

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