Soror, Sameh H. and Verma, V. and Rao, Ren and Rasool, Shafaq and Koul, S. and Qazi, G. N. and Cullum, John (2007) A cold-active esterase of Streptomyces coelicolor A3(2): from genome sequence to enzyme activity. Journal of Industrial Microbiology & Biotechnology, 34 (8). pp. 525-531. ISSN 1367-5435

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The genome sequence of Streptomyces coelicolor A3(2) contains 51 putative lipase and esterase genes mostly of unknown function. The gene estB (locus SCO 6966) was expressed as a His-tagged protein in E. coli. Esterase B was active at low temperatures exerting its maximum activity at 30°C and retaining more than 25% of its activity at 4°C. The optimum pH was 8–8.5. The enzyme was active against short synthetic p-nitrophenylesters (C2–C10) with maximum activity towards the acetate ester (C2). The esterase was tested on 13 series of racemic esters of potential interest for the synthesis of chiral pharmaceutical compounds. 4 of the series were substrates and a modestdegree of enantioselectivity was observed (enantiomeric ratios of 1.1–1.9).

Item Type: Article
Subjects: Biological Sciences
Depositing User: Mr. Amit Nargotra
Date Deposited: 19 Dec 2011 07:11
Last Modified: 19 Dec 2011 07:11

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